Indirect immunofluorescence assay for antibody to germ tube of Candida albicans--a new diagnostic test.

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Indirect immunofluorescence assay for antibody to germ tube of Candida albicans--a new diagnostic test.

Indirect immunofluorescent and agglutination assay were used to study the anti-Candida albicans reactivities in the serum of 13 normal subjects and 14 patients infected with C. albicans. A significant increase in anti-C. albicans seroreactivity was observed during infection with this organism but the increase in the anti-germ tube immunofluorescence titre was the more marked. It is evident that...

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New monoclonal antibody specific for Candida albicans germ tube.

Hydrophobic components of the germ tube of the dimorphic pathogenic fungus Candida albicans were used as immunogens to prepare monoclonal antibodies (MAbs). Among the resulting MAbs, one (MAb 16B1-F10) was shown by indirect immunofluorescence to be specific to the surface of the mycelium phase of the C. albicans and C. stellatoidea species. No labeling of any other genera and Candida species te...

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detection of antibodies to candida albicans germ tube by immunofluorescence in immunosuppressed mice with experimental systemic candidiasis

the increasing incidence of systemic candidiasis, which parallels the use of invasive and immunosuppressive medical procedures, necessitates development of rapid and cost effective tests for diagnosis of systemic candidiasis. therefore in this study 85 mice were first immunosuppressed by cyclophosphamide and then infected by candida albicans ncpf 3153. other 85 mice were employed as control. th...

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Clinical factors associated with a Candida albicans Germ Tube Antibody positive test in Intensive Care Unit patients

BACKGROUND Poor outcomes of invasive candidiasis (IC) are associated with the difficulty in establishing the microbiological diagnosis at an early stage. New scores and laboratory tests have been developed in order to make an early therapeutic intervention in an attempt to reduce the high mortality associated with invasive fungal infections. Candida albicans IFA IgG has been recently commercial...

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Kinetic patterns of Candida albicans germ tube antibody in critically ill patients: influence on mortality.

The influence of kinetic patterns of Candida albicans germ tube antibodies (CAGTA) on mortality was analyzed in six intensive care units. Statistically significant lower mortality rates were found in patients with patterns of increasing CAGTA titers who had been treated with antifungal agents. Thus, antifungal treatment should be considered when CAGTA titers are increasing in critically ill pat...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1976

ISSN: 0021-9746

DOI: 10.1136/jcp.29.11.1007